Figure 1. Western blot analysis of MCEMP1 using anti-MCEMP1 antibody (A17113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCEMP1 antigen affinity purified polyclonal antibody (Catalog # A17113-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCEMP1 at approximately 28 kDa. The expected band size for MCEMP1 is at 21 kDa.
Figure 1. Western blot analysis of MCEMP1 using anti-MCEMP1 antibody (A17113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCEMP1 antigen affinity purified polyclonal antibody (Catalog # A17113-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCEMP1 at approximately 28 kDa. The expected band size for MCEMP1 is at 21 kDa.
Anti-MCEMP1 Antibody Picoband(r)
A17113-1-PE
TargetMCEMP1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-MCEMP1 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID199675
- Target nameMCEMP1
- Target descriptionmast cell expressed membrane protein 1
- Target synonymsC19orf59; epididymis secretory sperm binding protein; mast cell-expressed membrane protein 1
- Protein IDQ8IX19
- Protein NameMast cell-expressed membrane protein 1
- Scientific DescriptionBoster Bio Anti-MCEMP1 Antibody Picoband® catalog # A17113-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203