Western blot analysis of MCM7 expression in HeLa cell lysate using anti-MCM7 antibody (M01649-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # M01649-1) at 0.5 ug/mL overnight at 4
Anti-MCM7 Monoclonal Antibody
M01649-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMCM7
Overview
- SupplierBoster Bio
- Product NameAnti-MCM7 Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBA-13
- FormulationLiquid
- Gene ID4176
- Target nameMCM7
- Target descriptionminichromosome maintenance complex component 7
- Target synonymsCDC47; CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; MCM2; minichromosome maintenance deficient 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104; protein phosphatase 1, regulatory subunit 104
- HostRabbit
- IsotypeIgG
- Protein IDP33993
- Protein NameDNA replication licensing factor MCM7
- Scientific DescriptionBoster Bio Anti-MCM7 Monoclonal Antibody catalog # M01649-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203