Figure 1. Western blot analysis of MCM8 using anti-MCM8 antibody (PB9722). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM8 antigen affinity purified polyclonal antibody (Catalog # PB9722) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM8 at approximately 94 kDa. The expected band size for MCM8 is at 94 kDa.
Figure 1. Western blot analysis of MCM8 using anti-MCM8 antibody (PB9722). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM8 antigen affinity purified polyclonal antibody (Catalog # PB9722) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM8 at approximately 94 kDa. The expected band size for MCM8 is at 94 kDa.
Anti-MCM8 Antibody Picoband(r)
PB9722
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human
TargetMCM8
Overview
- SupplierBoster Bio
- Product NameAnti-MCM8 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID84515
- Target nameMCM8
- Target descriptionminichromosome maintenance 8 homologous recombination repair factor
- Target synonymsC20orf154; dJ967N21.5; DNA helicase MCM8; DNA replication licensing factor MCM8; MCM8 minichromosome maintenance deficient 8; minichromosome maintenance complex component 8; POF10; REC homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ9UJA3
- Protein NameDNA helicase MCM8
- Scientific DescriptionBoster Bio Anti-MCM8 Antibody Picoband® catalog # PB9722. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203