Figure 1. Western blot analysis of MED4 using anti-MED4 antibody (A06467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody (Catalog # A06467-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 34KD.
Figure 1. Western blot analysis of MED4 using anti-MED4 antibody (A06467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody (Catalog # A06467-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 34KD.
Anti-MED4 Antibody Picoband(r)
A06467-2-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMED4
Overview
- SupplierBoster Bio
- Product NameAnti-MED4 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID29079
- Target nameMED4
- Target descriptionmediator complex subunit 4
- Target synonymsactivator-recruited cofactor 36 kDa component; ARC36; DRIP36; HSPC126; mediator of RNA polymerase II transcription subunit 4; mediator, 34-kD subunit, homolog; TRAP36; VDRIP; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein complex 36 kDa component
- HostRabbit
- IsotypeIgG
- Protein IDQ9NPJ6
- Protein NameMediator of RNA polymerase II transcription subunit 4
- Scientific DescriptionBoster Bio Anti-MED4 Antibody Picoband® catalog # A06467-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203