Figure 1. Western blot analysis of MEKK3/MAP3K3 using anti-MEKK3/MAP3K3 antibody (PA1924). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK3/MAP3K3 antigen affinity purified polyclonal antibody (Catalog # PA1924) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK3/MAP3K3 at approximately 71 kDa. The expected band size for MEKK3/MAP3K3 is at 71 kDa.
Figure 1. Western blot analysis of MEKK3/MAP3K3 using anti-MEKK3/MAP3K3 antibody (PA1924). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK3/MAP3K3 antigen affinity purified polyclonal antibody (Catalog # PA1924) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK3/MAP3K3 at approximately 71 kDa. The expected band size for MEKK3/MAP3K3 is at 71 kDa.
Anti-MEKK3/MAP3K3 Antibody Picoband(r)
PA1924
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMAP3K3
Overview
- SupplierBoster Bio
- Product NameAnti-MEKK3 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for MAP3K3 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID4215
- Target nameMAP3K3
- Target descriptionmitogen-activated protein kinase kinase kinase 3
- Target synonymsMAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; mitogen-activated protein kinase kinase kinase 3
- HostRabbit
- IsotypeIgG
- Protein IDQ99759
- Protein NameMitogen-activated protein kinase kinase kinase 3
- Scientific DescriptionBoster Bio Anti-MEKK3/MAP3K3 Antibody catalog # PA1924. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203