Anti-Mesothelin [C10]

Absolute Antibody

Anti-Mesothelin [C10]

9

This antibody can recognize and bind human mesothelin.

This antibody is recommended to be used for detection of mesothelin in biological samples. This high affinity anti-mesothelin antibody is derived from its parent antibody SS scFv by incorporating S92G-G93S-Y94H mutations in the light chain. The resulting antibody C10 has a 55 times higher affinity towards mesothelin in an ELISA as compared to its parent antibody. The binding characteristics and affinity of the antibody for mesothelin was determined by surface plasmon resonance (BIAcore assay). C10 binds mesothelin with an affinity of Kd = 0.2 nM. Immunoconjugates with C10 as targeting agent and Pseudomonas exotoxin A (PE38) as immunotoxin can be used for identifying samples containing mesothelin through immunohistochemical assays. The cytotoxic activity of the immunotoxin was also checked on antigen positive A1847 cells (PMID: 10385321, 9435250).

ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

C10

MSLN

mesothelin

CAK1 antigen; megakaryocyte potentiating factor; mesothelin; MPF; pre-pro-megakaryocyte-potentiating factor; SMRP; soluble MPF mesothelin related protein

Rabbit

IgG

Mesothelin

This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203