Anti-Mesothelin [K1]

Absolute Antibody

Anti-Mesothelin [K1]

7

This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.

This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Other Application, RadioImmunoAssay

IF; IHC; RIA; WB; ELISA; SPR

Research Use Only

Monoclonal

K1

MSLN

mesothelin

CAK1 antigen; megakaryocyte potentiating factor; mesothelin; MPF; pre-pro-megakaryocyte-potentiating factor; SMRP; soluble MPF mesothelin related protein

Rabbit

IgG

Mesothelin

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey

Human, Cynomologous monkey

The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.

-20°C,2°C to 8°C

12352203