Figure 1. Western blot analysis of MUT using anti-MUT antibody (A01065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human K562 whole cell lysates, lane 2: huam CACO-2 whole cell lysates, lane 3: rat liver tissue lysates, lane 4: rat kidney tissue lysates, lane 5: rat heart tissue lysates, lane 6: mouse liver tissue lysates, lane 7: mouse kidney tissue lysates, lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUT antigen affinity purified polyclonal antibody (Catalog # A01065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUT at approximately 83KD. The expected band size for MUT is at 83KD.
Figure 1. Western blot analysis of MUT using anti-MUT antibody (A01065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human K562 whole cell lysates, lane 2: huam CACO-2 whole cell lysates, lane 3: rat liver tissue lysates, lane 4: rat kidney tissue lysates, lane 5: rat heart tissue lysates, lane 6: mouse liver tissue lysates, lane 7: mouse kidney tissue lysates, lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUT antigen affinity purified polyclonal antibody (Catalog # A01065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUT at approximately 83KD. The expected band size for MUT is at 83KD.
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband(r)
A01065-10UG
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMMUT
Overview
- SupplierBoster Bio
- Product NameAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID4594
- Target nameMMUT
- Target descriptionmethylmalonyl-CoA mutase
- Target synonymsMCM; methylmalonyl Coenzyme A mutase; methylmalonyl-CoA isomerase; methylmalonyl-CoA mutase c.*192delA; methylmalonyl-CoA mutase c.*51C>G; methylmalonyl-CoA mutase variant c.1495G>A; methylmalonyl-CoA mutase variant c.2011A>G; methylmalonyl-CoA mutase variant c.2150G>T; methylmalonyl-CoA mutase variant c.322C>T; methylmalonyl-CoA mutase variant c.613_615delGAA; methylmalonyl-CoA mutase variant c.636G>A; methylmalonyl-CoA mutase variant c.643G>A; methylmalonyl-CoA mutase, mitochondrial; MUT; mutant methylmalonyl CoA mutase; truncated methylmalonyl CoA mutase; truncated methylmalonyl-CoA mutase variant c.1420C>T; truncated methylmalonyl-CoA mutase variant c.2179C>T; truncated methylmalonyl-CoA mutase variant c.91C>T
- HostRabbit
- IsotypeIgG
- Protein IDP22033
- Protein NameMethylmalonyl-CoA mutase, mitochondrial
- Scientific DescriptionBoster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® catalog # A01065. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203