Figure 1. Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (Catalog # PB9723) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 17KD. The expected band size for MGST1 is at 17KD.
Figure 1. Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (Catalog # PB9723) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 17KD. The expected band size for MGST1 is at 17KD.
Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband(r)
PB9723
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMGST1
Overview
- SupplierBoster Bio
- Product NameAnti-MGST1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID4257
- Target nameMGST1
- Target descriptionmicrosomal glutathione S-transferase 1
- Target synonymsglutathione S-transferase 12; GST12; MGST; MGST-I; microsomal glutathione S-transferase 1; microsomal GST-1; microsomal GST-I
- HostRabbit
- IsotypeIgG
- Protein IDP10620
- Protein NameMicrosomal glutathione S-transferase 1
- Scientific DescriptionBoster Bio Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband® catalog # PB9723. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203