Anti-MHC I [R1-21.2]

Absolute Antibody

Anti-MHC I [R1-21.2]

7

This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of

This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvs et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).

ImmunoPrecipitation, ImmunoHistoChemistry, RadioImmunoAssay

IHC; IP; radioimmunoassay

Research Use Only

Monoclonal

R1-21.2

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.

-20°C,2°C to 8°C

12352203