Anti-MHC II [OX-6]

Absolute Antibody

Anti-MHC II [OX-6]

7

This antibody recognises a monomorphic determinant of the rat MHC II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s]

This antibody binds to the rat MHC II (I-A) antigen, and cross-reacts with mouse I-A[k] and I-A[s]. Consequently, this antibody can be used to assess the tissue distribution of rat Ia antigens, indicating high Ia antigen expression on peripheral B lymphocytes, and significantly lower expression on thymocytes and T lymphocytes (McMaster, 1979).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, RadioImmunoAssay

RIA; IP; IF; WB; FC; IHC; ICC

Research Use Only

Monoclonal

OX-6

Rat

IgG2a

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse, Rat

Rat, Mouse

This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice.

-20°C,2°C to 8°C

12352203