Anti-Migis-alpha [29C11]

Absolute Antibody

Anti-Migis-alpha [29C11]

7

Membrane bound IgA is associated with Igalpha/Igbeta as the B cell receptor (BCR) complex on mIgA-expressing B cells. The alpha chain of mIgA (malpha) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-alpha and to various recombinant proteins containing migis-alpha as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).

ImmunoPrecipitation, ELISA

ELISA; IP

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203