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anti-MMP13 antibody

ARG41636
Arigo Biolaboratories
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMmp13
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-MMP13 antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID171052
  • Target name
    Mmp13
  • Target description
    matrix metallopeptidase 13
  • Target synonyms
    collagenase 3; interstitial collagenase; matrix metalloproteinase-13; MMP-13; UMRCASE
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Figure 1. Western blot analysis of MMP13 using anti-MMP13 antibody (A00420-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human U2OS whole cell lysates Lane 4: human HEK293 whole cell lysates Lane 5: rat testicular tissue lysates Lane 6: mouse testicular tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody (Catalog # A00420-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54KD. The expected band size for MMP13 is at 54KD.
Product group Antibodies
Boster Bio
Anti-MMP13 Antibody Picoband(r)A00420-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMmp13
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