Figure 1. Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB10008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB10008) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa, 92 kDa. The expected band size for MMP-9 is at 79 kDa.
Figure 1. Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB10008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB10008) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa, 92 kDa. The expected band size for MMP-9 is at 79 kDa.
Anti-MMP9 Antibody Picoband(r)
PB10008-10UG
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetMmp9
Overview
- SupplierBoster Bio
- Product NameAnti-MMP9 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID81687
- Target nameMmp9
- Target descriptionmatrix metallopeptidase 9
- Target synonyms92 kDa gelatinase; 92-kDa type IV collagenase; gelatinase B; GELB; matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); matrix metalloproteinase-9; MMP-9
- HostRabbit
- IsotypeIgG
- Protein IDP50282
- Protein NameMatrix metalloproteinase-9
- Scientific DescriptionBoster Bio Anti-MMP9 Antibody Picoband® catalog # PB10008. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203