Flow Cytometry analysis of U251 cells using anti-Myelin oligodendrocyte glycoprotein antibody (A03294). Overlay histogram showing U251 cells stained with A03294 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.