Figure 1. Western blot analysis of MOSPD2 using anti-MOSPD2 antibody (A16695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOSPD2 antigen affinity purified polyclonal antibody (Catalog # A16695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOSPD2 at approximately 60 kDa. The expected band size for MOSPD2 is at 60 kDa.
Figure 1. Western blot analysis of MOSPD2 using anti-MOSPD2 antibody (A16695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOSPD2 antigen affinity purified polyclonal antibody (Catalog # A16695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOSPD2 at approximately 60 kDa. The expected band size for MOSPD2 is at 60 kDa.
Anti-MOSPD2 Antibody Picoband(r)
A16695-1-BIOTIN
TargetMOSPD2
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-MOSPD2 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID158747
- Target nameMOSPD2
- Target descriptionmotile sperm domain containing 2
- Target synonymsmotile sperm domain-containing protein 2
- Protein IDQ8NHP6
- Protein NameMotile sperm domain-containing protein 2
- Scientific DescriptionBoster Bio Anti-MOSPD2 Antibody Picoband® catalog # A16695-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203