Anti-Mouse Nidogen/enactin [JF4 (5G12-D4-E3)]

Absolute Antibody

Anti-Mouse Nidogen/enactin [JF4 (5G12-D4-E3)]

7

This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin.

The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). While researching nidogen in healthy human cartilage and in late-stage osteoarthritis cartilage an inhibition assay was preformed using the mouse version of this antibody. In short, wells were coated with either nidogen-1 or nidogen-2 to which this antibody was added. Then, chondrocytes were added and their adhesion to the nidogen was tested. 30-40% of chondrocytes showed adhesion to nidogen-1. (Kruegel et al, 2008; pmid:18438862). Tissue sections from Female New Mexico Research Institute (NMRI) mice were stained for immunohistochemistry using this antibody as a positive control. This experiment was preformed to assess the binding between laminin and nidogen (Gersdorff et al, 2005; pmid:15824114). Tissue sections from aborted human embryos were stained with the mouse version of this antibody to be used for immunohistochemistry (Miosge et al, 2001; pmid:12005023). In research on osteoarthritis multiple experiments were preformed using the mouse version of this antibody. First, tissues from DDR-1 KO mice were stained for immunohistochemistry using the mouse version of this antibody. Second, immunoblotting was preformed on proteins extracted from DDR-1 KO mice. Third, this antibody was used to stain cell cultures taken from TMJ cartilage tissue of 9-week-old DDR-1 KO mice and their WT littermates. Finally, the cell cultures taken from TMJ cartilage tissue of 9-week-old DDR-1 KO mice and their WT littermates were used for flow cytometry and stained with this antibody (Schminke et al, 2014; pmid:23912900). In research on nidogen-2, the mouse version of this antibody was used to immunolocalize nidogen-1 in both immunofluorescence and immunohistochemistry on cells and tissues from mice with a mutated nidogen-2 gene (Schymeinsky et al, 2002; pmid:12215539).

Functional Assay, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

ELISA; SPR; IHC; WB; IF; FC; functional assay

Research Use Only

Monoclonal

JF4 (5G12-D4-E3)

Nid1

nidogen 1

A630025O17; enta; entact; entactin; entactin 1; entactin-1; Nid; NID-1; nidog; Nidogen precursor (Entactin); nidogen-1

Human

IgG1

Nidogen-1

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

Human, Mouse

This antibody was raised by immunizing a rat with he G2 region of mouse nidogen that is able to block its binding to laminin.

-20°C,2°C to 8°C

12352203