Western blot analysis of MRP1 using anti-MRP1 antibody (A00872). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates, lane 2: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP1 antigen affinity purified polyclonal antibody (Catalog # A00872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP1 at approximately 220KD. The expected band size for MRP1 is at 220KD.
Western blot analysis of MRP1 using anti-MRP1 antibody (A00872). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates, lane 2: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP1 antigen affinity purified polyclonal antibody (Catalog # A00872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP1 at approximately 220KD. The expected band size for MRP1 is at 220KD.
Anti-MRP1/ABCC1 Antibody Picoband(r)
A00872
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetABCC1
Overview
- SupplierBoster Bio
- Product NameAnti-MRP1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID4363
- Target nameABCC1
- Target descriptionATP binding cassette subfamily C member 1
- Target synonymsABC29; ABCC; ATP-binding cassette transporter variant ABCC1delta-ex13; ATP-binding cassette transporter variant ABCC1delta-ex13&14; ATP-binding cassette transporter variant ABCC1delta-ex25; ATP-binding cassette transporter variant ABCC1delta-ex25&26; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; DFNA77; glutathione S-conjugate export pump; glutathione-S-conjugate-translocating ATPase ABCC1; GS-X; leukotriene C(4) transporter; LTC4 transporter; MRP; MRP1; multidrug resistance-associated protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP33527
- Protein NameMultidrug resistance-associated protein 1
- Scientific DescriptionBoster Bio Anti-MRP1/ABCC1 Antibody Picoband® catalog # A00872. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203