Western blot analysis of ABCC2 using anti-ABCC2 antibody (A00974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCC2 at approximately 190-250KD. The expected band size for ABCC2 is at 174KD.
Western blot analysis of ABCC2 using anti-ABCC2 antibody (A00974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCC2 at approximately 190-250KD. The expected band size for ABCC2 is at 174KD.
Anti-MRP2/ABCC2 Antibody Picoband(r)
A00974-1
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetABCC2
Overview
- SupplierBoster Bio
- Product NameAnti-MRP2/ABCC2 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID1244
- Target nameABCC2
- Target descriptionATP binding cassette subfamily C member 2
- Target synonymsABC30; ATP-binding cassette sub-family C member 2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; canalicular multidrug resistance protein; canalicular multispecific organic anion transporter 1; CMOAT; cMRP; DJS; MRP2; multidrug resistance-associated protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ92887
- Protein NameCanalicular multispecific organic anion transporter 1
- Scientific DescriptionBoster Bio Anti-MRP2/ABCC2 Antibody Picoband® catalog # A00974-1. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203