Anti-MSCP [LC007]

Absolute Antibody

Anti-MSCP [LC007]

7

This antibody is specific for repeat 14 of MSCP or for an epitope that is only reconstituted when repeat 14 is present. It also binds to cynomolgus MSCP. MSCP is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythro

The binding epitope of this antibody was mapped by preforming flow cytometry on 4 different constructs. These were: construct 1 containing CSPG repeat 15, construct 2 containing CSPG repeat 14-15, construct 3 containing CSPG repeat 13-15, and construct 4 containing CSPG repeat 12-15 (US2014242083). Flow cytomtery was used to determine whether the human version of this antibody has cross-reactivity with human and cynomolgus antigen. The cells used were HEK-EBNA expressing either a construct containing the C-terminal part of the cynomolgus MCSP protein, a signal peptide for secretion and a N-terminal FLAG-tag or the human variant of this construct (US2014242083). Further, lysis of Colo38 human malignant melanoma cells by human lymphocytes was assessed using the glycoengineerd mouse version of this antibody (US2014242083). Co-culturing CD16-CAR T cells with A375 cells in presence of the human version of this antibody resulted in a dose-dependent increase in activation for the human version of this antibody (Rataj et al, 2018; pmid:30429531).

Functional Assay, Flow Cytometry

FC; functional assay

Research Use Only

Monoclonal

LC007

SLC25A37

solute carrier family 25 member 37

HT015; MFRN; MFRN1; mitochondrial iron transporter 1; mitochondrial solute carrier protein; mitoferrin; mitoferrin-1; MSC; MSCP; predicted protein of HQ2217; PRO1278; PRO1584; PRO2217; solute carrier family 25 (mitochondrial iron transporter), member 37

Human

IgG1

Mitoferrin-1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey

Human, Monkey

The original mouse antibody was raised by immunizing Balb/c mice with a synthetic peptide corresponding to aa 2177-2221 of the human MCSP sequence coupled to KLH.

-20°C,2°C to 8°C

12352203