Anti MSR-A / CD204 mAb (Clone SRA-E5, IgG1)

Cosmo Bio USA

Anti MSR-A / CD204 mAb (Clone SRA-E5, IgG1)

16

Research Use Only

Macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules that are implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediated uptake of modified low density lipoproteins (LDL). MSR-As possess a wide range of ligand-binding specificities and recognize a variety of molecules such as modified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGE), polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide and lipoteicoic acid. This antidody was produced from the mouse immunized with recombinant protein of human type I MSR-A ,and has been proved to be useful for the immunoblotting and immunohistochemistry. This antibody is useful tools for the study of MSR in atherogenesis and various other pathological conditions in humans and animal species. Package Size 50 g (200 l / vial) Format Mouse monoclonal antibody 0.25mg/ml Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat Storage Store below -20 Clone No. SRA-E5 Subclass IgG1 Purification method The spleen cells from mouse, immunized with recombinant protein of human type I SR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography. Working dilution for immunohistochemistry: 10 g/ml , for immunoblotting : 2 g/ml Antigen retrieval (microwave 10 min , 0.01M citrate buffer, ph2.0) is recommended for paraffin sections. Reference 1. Takeya M., Tomokiyo R., Jinnouchi K., Sakaguchi H., et al: Macrophage Scavenger Receptors: Structure, Function and Tissue Distribution: Acta Histochem. Cytochem. 32(1):47-51,1999 2. Tomokiyo R., Jinnouchi K., Honda M., Wada Y., Hanada N., Hiraoka T., Suzuki H., Kodama T., Takahashi K., Takeya M.: Production, characterization, and interspecies reactivities of monoclonal antibodies against human class A macrophage scavenger receptors: Atherosclerosis, 2001, in press. 3. Takeya M., Tomokiyo R., Jinnouchi K. Honda M. et al: CD204: Macrophage scavenger receptor, a new differentiation marker for macrophages: part 9 Myeloid Cells, New CD Antigens, Leucocyte Typing , Oxford Univ. Press, 2001, in press.

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