Anti-MUC1 [HMFG1 (1.10.F3)]

Absolute Antibody

Anti-MUC1 [HMFG1 (1.10.F3)]

7

This antibody binds the MUC1, a tumor associated antigen expressed on many breast cancer cells. This antibody does not show reactivity towards fibroblasts, lymphoblastoid cells, and a large number of epithelial cell lines of non-breast origin. This antibo

This antibody reacts with most of the breast cancer cells lines and with epithelial cells of human milk. The antibody also reacts with neoplasms of epithelial origin, particularly carcinomas of breast, ovary, cervix, bronchus and gastrointestinal tract. This antibody was used for the study of expression of epithelial antigen in normal and disordered cervical squamous epithelium using immunohistological methods (PMID: 6315048). This antibody can recognize the epithelial membrane antigen in a radioimmunoassay. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution (PMID: 6086018). HMFG1 and HMFG2 recognize different epitopes of the millk fat globule (PMID: 6086018, 2424847). This antibody was used for the study of tumor associated antigenic epitopes purified from human skimmed milk using immunoaffinity chromatography, immunoprecipitation and immunoblot techniques (PMID: 2443241). The reshaped humanized antibody, HuHMFGl, showed no difference in relative affinity for its antigen compared with the parent mouse HMFG1 (PMID: 7682986). This mAb reacts with P1-24 synthetic peptide (PDTRPAPGSTAPPAHGVTSAPDTR) representing the repeating amino acid sequence of the mucin core protein and also reacted strongly with ovarian carcinoma-associated sebaceous gland antigen (SGA) by direct binding and in a sandwich ELISA assay with OM-1 as the capture Mab (PMID: 1697426).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay

IHC; RIA; IF; IP; WB; ELISA

Research Use Only

Monoclonal

HMFG1 (1.10.F3)

MUC1

mucin 1, cell surface associated

ADMCKD; ADMCKD1; ADTKD2; breast carcinoma-associated antigen DF3; CA 15-3; cancer antigen 15-3; carcinoma-associated mucin; CD227; EMA; episialin; H23 antigen; H23AG; KL-6; krebs von den Lungen-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; mucin 1, transmembrane; mucin-1; peanut-reactive urinary mucin; PEM; PEMT; polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen

Human

IgG1

Mucin-1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing mice with human mammary milk fat globule.

-20°C,2°C to 8°C

12352203