Anti-MUC1 [Mc5]

Absolute Antibody

Anti-MUC1 [Mc5]

7

This antibody is specific for the amino acid sequence DTRPAP of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial an

In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992; pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 ; pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006; pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008; pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008; pmid:18821582). While assesing a panel of 56 anti MUC1 antibodies for their ability to detect cancer the mouse version of this antibody was used for an immunoradiometric assay on a serum made from sera from 10 different patients with breast, ovarian or colon cancer (Norum et al, 1998; pmid:9422099). To more accurately determine the epitope of this antibody, an ELISA was preformed using the murine version of this antibody on different small pepetides from the MUC1 protein (Karsten et al, 2004; pmid:15115750).

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

IP; ELISA; WB; IHC; IRA

Research Use Only

Monoclonal

Mc5

MUC1

mucin 1, cell surface associated

ADMCKD; ADMCKD1; ADTKD2; breast carcinoma-associated antigen DF3; CA 15-3; cancer antigen 15-3; carcinoma-associated mucin; CD227; EMA; episialin; H23 antigen; H23AG; KL-6; krebs von den Lungen-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; mucin 1, transmembrane; mucin-1; peanut-reactive urinary mucin; PEM; PEMT; polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen

Mouse

IgG2a

Mucin-1

Human

Human

Human breast epithelial mucin.

-20°C,2°C to 8°C

12352203