Anti-Mycobacterium avium cell lysate [SurfS2.2]

Absolute Antibody

Anti-Mycobacterium avium cell lysate [SurfS2.2]

7

The scFv version of this antibody was immunoblotted on M. avium subsp. paratuberculosis lysate. The result showed a positive reaction. Furthermore, flow cytometry on cobacterium sp. surface antigen was performed using this antibody, the antibody showed binding to M. avium subsp. avium cells. To determine whether surface-bound SurfS1.2 or SurfS2.2 could influence mycobacterial growth, M. avium subsp. avium was grown in Middlebrook broth, the antibody prevented clumping in M. avium subsp. avium cells. The antibody was also used to magnetically separate M. avium subsp. paratuberculosis from faecal samples (Berger et al, 2006; pmid:16960114).

Functional Assay, Flow Cytometry, Western Blot

Research Use Only

This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original scFv format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203