Anti-NeuGcGM3 [P3], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-NeuGcGM3 [P3], Human IgG1, Fc Silent(tm), kappa

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This antibody is specific for the N-glycolyl neuraminic acid (NeuGc) linked to the internal galactose of gangliosides, showing no cross-reactivity to the highly similar N-acetylated (NeuAc) gangliosides that are common cellular antigens in humans. This an

The original antibody (mouse IgM) was generated and screened via ELISA against for gangliosides of interest. This antibody was used for tumor recognition in IHC, showing a strong positive reaction in ductal infiltrating breast carcinoma, breast cancer metastasis in lymph nodes, and breast adenosis tissue, with no cross-reactivity to other malignant or benign tumors. Additionally, it was used in in vivo experiments with Balb/c mice to stimulate immune responses, generating a significant IgG-type anti-idiotypic (Ab2) response, which increased when conjugated to KLH and administered with adjuvants. This antibody also produced an anti-anti-idiotypic (Ab1) response, demonstrating its ability to connect to both T and B cell networks and generate a specific antigen-independent immune response (EP0657471B1). This antibody was used as a positive control in an in vitro and in vivo study (Carr et al., 2002; PMID: 12573110). A chimeric(humanized; human IgG1) variant of this antibody was created (chP3), and the crystal structure of its Fab was solved (Talavera et al., 2009; PMID: 19748674).

ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

P3

Human

IgG1

This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgM format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203