
Figure 1. Western blot analysis of Neurocan using anti-Neurocan antibody (A06700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody (Catalog # A06700-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
Anti-Neurocan/NCAN Antibody Picoband(r)
A06700-1-IFLUOR647
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCAN
Overview
- SupplierBoster Bio
- Product NameAnti-Neurocan/NCAN Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID1463
- Target nameNCAN
- Target descriptionneurocan
- Target synonymschondroitin sulfate proteoglycan 3 (neurocan); CSPG3; neurocan core protein; neurocan proteoglycan
- HostRabbit
- IsotypeIgG
- Protein IDO14594
- Protein NameNeurocan core protein
- Scientific DescriptionBoster Bio Anti-Neurocan/NCAN Antibody Picoband® catalog # A06700-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203