Anti-NKG2D [KYK-2]

Absolute Antibody

Anti-NKG2D [KYK-2]

9

This antibody bind human NKG2D. Natural killer (NK) cells serve as key innate effectors and their activity has been considered a prognostic biomarker in diverse human diseases. NKG2d functions as an activating and costimulatory receptor involved in immuno

The binding of this antibody to NKG2D expressed on subpopulations of human peripheral blood mononuclear cells (PBMC) was done using flow cytometry. This antibody was not capable of binding mouse NKG2D. The IgG1 version of this antibody is reported to show dual antagonistic and agonistic activity. It was reported that soluble KYK-2.0 IgG1 exhibits antagonistic activity through interfering with effector cell to target cell recognition mediated by NKG2D receptor/ligand interactions. KYK-2.0 IgG1 in solution significantly blocked the acquired cytolytic activity of ex vivo expanded human NK cells. The agonistic activity of this antibody was determined using a degranulation assay. It was shown that immobilized KYK-2.0 IgG1 potently induced NK cell degranulation in PBMC from 4 different healthy donors (PMID: 18809410). This antibody was also used in the generation of a bispecific antibody targeting NKG2D and 2B4 receptors. The combination mounts selective cytotoxicity and IFN-gamma production of NK cells and helps in assessing NK cell functions (PMID: 34486371). This antibody was also used in the generation of another bispecific antibody against KNG2B and ErbB2 (PMID: 34599028).

Flow Cytometry, ELISA, Other Application

Research Use Only

Monoclonal

KYK-2

KLRK1

killer cell lectin like receptor K1

CD314; D12S2489E; killer cell lectin-like receptor subfamily K, member 1; KLR; NK cell receptor D; NKG2D; NKG2-D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

Human

IgG1

NKG2-D type II integral membrane protein

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203