Anti-Notch 1 [WC613]

Absolute Antibody

Anti-Notch 1 [WC613]

9

This antibody recognizes and binds the ligand-binding region (LBD) of Notch 1.

This antibody is capable of immunoprecipitating Notch 1 but not Notch 4. The binding affinity of this antibody towards Notch1 was checked using ELISA. The binding of this antibody to full length Notch 1 overexpressed on HEK cells was confirm using flow cytometry. Flow cytometry was also used to study the expression of Notch 1 on cancer cell lines like LS-1034, TALL-1, BxPC3, and Colo_205. The antagonistic activity of WC613 was strongly dependent on the particular ligand-expressing cell line used to activate Notch1. This antibody competes with DLL4 for binding to Notch1. This antibody is capable of inhibiting Jag2 mediated Notch1 signalling with an EC50 value of 5+/-3 nM. This antibody completely inhibited Hes1 transactivation (PMID: 20161710).

Flow Cytometry, ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

WC613

NOTCH1

notch receptor 1

AOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1

Rabbit

IgG

Neurogenic locus notch homolog protein 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203