Anti-Notch 3 [hN3S2-160]

Absolute Antibody

Anti-Notch 3 [hN3S2-160]

7

This antibody binds human notch 3.

The original human IgG1 antibody hN3S2-160 binds notch 3 with an EC50 =0.06 nM. The binding affinity of this antibody for notch 3 was determined using ELISA. The binding of hN3S2-160 to notch 3 on 293 Flp-TRex cells was done using flow cytometry. This antibody is also capable of blocking notch 3 and DLL4 ligand interaction (WO2010141249).

Flow Cytometry, ELISA

ELISA; FC;

Research Use Only

Monoclonal

hN3S2-160

NOTCH3

notch receptor 3

CADASIL; CADASIL1; CASIL; IMF2; LMNS; neurogenic locus notch homolog protein 3; notch 3; Notch homolog 3

Mouse

IgG2b

Neurogenic locus notch homolog protein 3

This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was isolated from an scFv phage display library generated from BMV (bone marrow), CS (spleen) and DP47 (frame work based CS) and by panning against a fusion protein containing Notch3 extracellular fragment (amino acid 40-467) and human IgG1-Fc.

-20°C,2°C to 8°C

12352203