Anti-Occludin [1-3]

Absolute Antibody

Anti-Occludin [1-3]

9

This antibody is specific for the second extracellular loop (EL2) of OCLN.

The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibodys Kd for hOCLN is 0.580 +/- 0.031 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. Furthermore, other TJ proteins expressed in T84 cells - TRIC and ZO-2 - were found to be co-immunoprecipitated with OCLN by this antibody. Immunofluorescence (IF) staining of OCLN using clone 1-3 with a TJ marker protein ZO-1 in Caco-2 cells was co-localized with ZO-1 at cell-cell contact sites. This antibody was found to cross-react with crab-eating monkey (moOCLN), mouse (mOCLN), and rat (rOCLN) as determined by FC (Shimizu et al., 2019; PMID: 31077306).

Flow Cytometry, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

1-3

OCLN

occludin

BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1

Mouse

IgG2b

Occludin

Human, Monkey, Mouse, Rat

-20°C,2°C to 8°C

12352203