Anti-OPGL [18B2]

Absolute Antibody

Anti-OPGL [18B2]

9

This antibody is specific for human OPGL. In vivo experiments have shown cross reactivity with Cynomolgus Monkey OPGL.

The binding affinity of this antibodys original format (human IgG1) to hOPGL was confirmed by ELISA. Its binding kinetics to hOPGL 140 and hOPGL 158 was measured by SPR analysis; a KD of 0.211 nM was measured for hOPGL 140. This antibody was evaluated for its neutralizing activity against osteoclast formation induced by Osteoprotegerin ligand (OPGL) using RAW 264.7 cells, as measured by tartrate-resistant acid phosphatase (TRAP) activity; it inhibited osteoclast formation in a dose-dependent manner with an IC50 of 80 ng/ml. This antibodys pharmacokinetics and in vivo activity were assessed in cynomolgus monkeys - three female cynomolgus monkeys (not greater than five years of age, each weighing between 2 to 5 kgs) received a single subcutaneous dose of 1 mg/kg; serum samples were collected over 56 days to determine the levels of the antibody and analyze bone turnover markers such as serum N-telopeptide (serum N-Tx). The antibodys levels in the serum declined slowly over the testing period; the last measurement on day 56 showed a serum concentration of ~100 ng/ml. Serum N-Tx levels were greatly reduced compared to baseline immediately following injection (~minus 60%), further decreased to a minimum of ~minus 75% over a period of two weeks, after which they began to increase gradually but remained lower in a statistically significant manner than baseline with a final measurement of ~minus 25% on day 56 (US7718776B2).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

18B2

TNFSF11

TNF superfamily member 11

CD254; hRANKL2; ODF; OPGL; OPTB2; osteoclast differentiation factor; osteoprotegerin ligand; RANKL; receptor activator of nuclear factor kappa B ligand; sOdf; TNF-related activation-induced cytokine; TNLG6B; TRANCE; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11

Rabbit

IgG

Tumor necrosis factor ligand superfamily member 11

Human, Monkey

-20°C,2°C to 8°C

12352203