Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Osteocalcin/BGLAP Antibody
PB9918
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBGLAP
Overview
- SupplierBoster Bio
- Product NameAnti-Osteocalcin Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID632
- Target nameBGLAP
- Target descriptionbone gamma-carboxyglutamate protein
- Target synonymsBGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin
- HostRabbit
- IsotypeIgG
- Protein IDP02818
- Protein NameOsteocalcin
- Scientific DescriptionBoster Bio Anti-Osteocalcin/BGLAP Antibody Picoband® catalog # PB9918. Tested in IHC applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates. Wang Y et al., 2013 May, BiomaterialsRead more