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Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9920). Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Osteocalcin Antibody (PB9920) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9920). Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Osteocalcin Antibody (PB9920) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9920). Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Osteocalcin Antibody (PB9920) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-Osteocalcin/BGLAP Antibody

Research Use Only
PB9920-FITC
Boster Bio
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetBglap
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Osteocalcin/BGLAP Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    FITC
  • Gene ID25295
  • Target name
    Bglap
  • Target description
    bone gamma-carboxyglutamate protein
  • Target synonyms
    Bglap2; Bgp; Bgpr; Bgpra; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); bone Gla-protein; gamma-carboxyglutamic acid-containing protein; osteocalcin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04640
  • Protein Name
    Osteocalcin
  • Scientific Description
    Boster Bio Anti-Osteocalcin/BGLAP Antibody Picoband® catalog # PB9920. Tested in Flow Cytometry, IHC applications. This antibody reacts with Rat.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203