Anti-osteosarcoma-associated cell membrane antigen [TP-1]

Absolute Antibody

Anti-osteosarcoma-associated cell membrane antigen [TP-1]

9

This osteosarcoma-specific antibody binds an epitope on a alkaline phosphatase isoform expressed on both canine and human osteosarcoma cells, but with very limited expression on normal cells and other cancer forms. It also reacts with some other major sub

In indirect immunofluorescence assays, this antibody has been show to bind to the surface of human osteosarcoma TPX cells, but not to autologous skin fibroblasts, non-sarcomatous tumor cells or peripheral blood lymphocytes (Bruland et al, 1986).In IHC analysis, this antibody has been shown to bind to osteosarcomas, along with other major human sarcoma subtypes, including synovial sarcomas, malignant fibrous histiocytomas, malignant hemangiopericytomas and chondrosarcomas. It does no react with liposarcomas and leio- and rhabdomyosarcomas, or non-sarcomatous malignancies or normal tissue (Bruland et al, 1986). This binding profile matches that of MAb TP-3. A radiolabelled Fab derived from this antibody localises to human osteogenic sarcoma xenografts after intravenous injection (Bruland et al, 1987). In a pilot study, this antibody showed potential as a immunoscintigraphic agent for the early detection of lung metastases in patients with bone sarcoma (Bruland et al, 1994).

ImmunoFluorescence, ImmunoHistoChemistry

Research Use Only

Monoclonal

TP-1

Canine

IgG2

This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Canine, Human

-20°C,2°C to 8°C

12352203