IHC analysis of CDH3 using anti-CDH3 antibody (PA1363). CDH3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of CDH3 using anti-CDH3 antibody (PA1363). CDH3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-P-Cadherin-3 CDH3-Antibody Picoband(r)
PA1363
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human
TargetCDH3
Overview
- SupplierBoster Bio
- Product NameAnti-P-Cadherin Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID1001
- Target nameCDH3
- Target descriptioncadherin 3
- Target synonymscadherin 3, type 1, P-cadherin (placental); cadherin-3; calcium-dependent adhesion protein, placental; CDHP; HJMD; PCAD
- HostRabbit
- IsotypeIgG
- Protein IDP22223
- Protein NameCadherin-3
- Scientific DescriptionBoster Bio Anti-P-Cadherin-3 CDH3-Antibody catalog # PA1363. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human
- Reactivity SupplierHuman, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203