Anti-p130/CAS [8G4]

Absolute Antibody

Anti-p130/CAS [8G4]

7

This antibody is specific for p130/CAS amino acids 576 to 796 and recognizes rat, mouse and human homologs. P130cas is a docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity).

The function of CAS in cell signaling was researched. For this a western blot was preformed on rat and murine fibroblasts, as well as human osteosarcoma, and COS-7 cells using the murine version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Furthermore with the same goal CE cells were immunostained using the mouse version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Immunoblotting was preformed on NIH3T3 cells using the mouse version of this antibody. This was done to research FAK phosphorylation (Lim et al, 2004; pmid:15123632). To assay the effect of PTP-PEST overexpression the mouse version of this antibody was used for immunoblotting on Bosc23 cells transfected with the gene encoding full-length human PTP-PEST (Garton and Tonks, 1999; pmid:9920935). In a study on CRKII as a possible target for cancer gene terapy the murine version of this antibody was used for immunoblotting on a human OSCC cell line (Yamada et al, 2011; pmid:21339045).

ImmunoFluorescence, Western Blot

WB; IF

Research Use Only

Monoclonal

8G4

Bcar1

BCAR1 scaffold protein, Cas family member

BCAR1, Cas family scaffold protein; BCAR1, Cas family scaffolding protein; breast cancer anti-estrogen resistance 1; breast cancer anti-estrogen resistance protein 1; Cas; Crkas; CRK-associated substrate; P130CAS; v-crk-associated tyrosine kinase substrate

Rabbit

IgG

Breast cancer anti-estrogen resistance protein 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse, Rat

human, mouse, rat

The original mouse version of this antibody was raised by immunizing a mosue with a GST fusion protein corresponding to residues 576-796 of rat p130 Cas.

-20°C,2°C to 8°C

12352203