Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody(M00145-3) CDKN1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1A Antibody (M00145-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody(M00145-3) CDKN1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1A Antibody (M00145-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
M00145-3
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDKN1A
Overview
- SupplierBoster Bio
- Product NameAnti-p21 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDACG-3
- FormulationLiquid
- Gene ID1026
- Target nameCDKN1A
- Target descriptioncyclin dependent kinase inhibitor 1A
- Target synonymsCAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CIP1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; P21; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
- HostRabbit
- IsotypeIgG
- Protein IDP38936
- Protein NameCyclin-dependent kinase inhibitor 1
- Scientific DescriptionBoster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-3. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203
References
- Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration. Chen Q et al., 2021 Apr 26, Cancer Cell IntRead more