Anti-p21 [Y13-238]

Absolute Antibody

Anti-p21 [Y13-238]

7

The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21s but not v-K-ras or rodent c-K-ras p21s. This antibody also recognizes human c-H-ras and c-K-ras p21s, but not human c-N-ras.

Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).

ImmunoPrecipitation

IP;

Research Use Only

Monoclonal

Y13-238

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

Human, Mouse

Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.

-20°C,2°C to 8°C

12352203