Anti-p21Ras [KGH-R1]

Absolute Antibody

Anti-p21Ras [KGH-R1]

9

This antibody recognizes human wild-type and mutant H-, K-, and N-Ras proteins. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). T

The binding reactivity of this antibody to H-p21Ras, K-p21Ras and N-p21Ras protein was determined using ELISA. This antibody was also capable of recognizing wild type and mutated ras protein from various normal and tumor cell lines using western blot and immunocytometry assay. This antibody presented strong immunoreactivity for 14 primary human cancer tissues but showed weak or negative immunoreactivity in the corresponding normal tissues. It was reported to bind positively with QGY7703, SMMC7721, HepG2: hepatocarcinoma; BGC853, MKN28: gastric cancer; HCT116: colorectal cancer; T24: bladder cancer; SKOV3: ovary cancer; MDA-MB-231, MDA-MB-435, MCF7: breast cancer; HeLa: cervical cancer; Hep2: laryngocarcinoma; C8166, K562 cell lines (PMID: 26897358). This antibody was also used in the immunohistochemical staining of FFPE section of colorectal tissues, colorectal inflammatory polyps, colorectal low-grade intraepithelial neoplasia, colorectal high-grade intraepithelial neoplasia, invasive colorectal carcinomas and corresponding adjacent tissues. The immunoreactivity was measured by evaluating percentage of positive cells and histological score (HSCORE) (PMID: 21138685). This antibody can also be used in the immunofluorescent staining of various normal and cancer tissues (PMID: 32664770).

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

KGH-R1

HRAS

HRas proto-oncogene, GTPase

C-BAS/HAS; C-HA-RAS1; c-has/bas p21 protein; C-H-RAS; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; HAMSV; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS1; H-RASIDX; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; RASH1; transformation gene: oncogene HAMSV; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Rabbit

IgG

GTPase NRas

Human

-20°C,2°C to 8°C

12352203