Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody(M00173-1) CDKN1B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1B Antibody (M00173-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-p27 KIP 1 Rabbit Monoclonal Antibody
M00173-1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-p27 KIP 1 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203