Western blot analysis of p38 MAPK expression in Jurkat cell lysate (M00176-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK14 monoclonal antibody (Catalog # M00176-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Western blot analysis of p38 MAPK expression in Jurkat cell lysate (M00176-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK14 monoclonal antibody (Catalog # M00176-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
M00176-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK14
Overview
- SupplierBoster Bio
- Product NameAnti-p38 MAPK Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEGC-13
- FormulationLiquid
- Gene ID1432
- Target nameMAPK14
- Target descriptionmitogen-activated protein kinase 14
- Target synonymsCSAID-binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; EXIP; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38 alpha; MAX-interacting protein 2; mitogen-activated protein kinase 14; mitogen-activated protein kinase p38 alpha; Mxi2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK2A; stress-activated protein kinase 2A
- HostRabbit
- IsotypeIgG
- Protein IDQ16539
- Protein NameMitogen-activated protein kinase 14
- Scientific DescriptionBoster Bio Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody catalog # M00176-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway. Wang X et al., 2018 Dec 1, ToxiconRead more