IHC analysis of p53 using anti-p53 antibody (MA1078). p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
IHC analysis of p53 using anti-p53 antibody (MA1078). p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-P53 Tp53 Monoclonal Antibody
MA1078
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTP53
Overview
- SupplierBoster Bio
- Product NameAnti-P53 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteOther applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, IHF, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBP53-12
- Concentration100 ug/ml
- FormulationLyophilized
- Gene ID7157
- Target nameTP53
- Target descriptiontumor protein p53
- Target synonymsantigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
- HostMouse
- IsotypeIgG2a
- Protein IDP10361
- Protein NameCellular tumor antigen p53
- Scientific DescriptionBoster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway. Yang P et al., 2022 Mar 9, J Agric Food ChemRead more
- Targeted inhibition of GRP78 by HA15 promotes apoptosis of lung cancer cells accompanied by ER stress and autophagy. Wu J et al., 2020 Nov 12, Biol OpenRead more
- YY1/BCCIP Coordinately Regulates P53-Responsive Element (p53RE)-Mediated Transactivation of p21(Waf1/Cip1). Sui Y et al., 2019 Apr 28, Int J Mol SciRead more
- The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death. Liu G et al., 2018 Jun 7, Cell Death DisRead more
- Knockdown of translationally controlled tumor protein inhibits growth, migration and invasion of lung cancer cells. Wang L et al., 2018 Jan 15, Life SciRead more
- The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum. Yin H et al., 2016 Jun 14, OncotargetRead more
- Dietary NiCl2 causes G2/M cell cycle arrest in the broilers kidney. Guo H et al., 2015 Nov 3, OncotargetRead more
- Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin. Liang S et al., 2015 Jan 20, OncotargetRead more