Figure 1. Western blot analysis of PACE4 using anti-PACE4 antibody (PB9769). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACE4 antigen affinity purified polyclonal antibody (Catalog # PB9769) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACE4 at approximately 80 kDa, 45/80/100 kDa. The expected band size for PACE4 is at 80 kDa, 45/80/100 kDa.
Figure 1. Western blot analysis of PACE4 using anti-PACE4 antibody (PB9769). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACE4 antigen affinity purified polyclonal antibody (Catalog # PB9769) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACE4 at approximately 80 kDa, 45/80/100 kDa. The expected band size for PACE4 is at 80 kDa, 45/80/100 kDa.
Anti-PACE4/PCSK6 Antibody Picoband(r)
PB9769
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetPCSK6
Overview
- SupplierBoster Bio
- Product NameAnti-PACE4 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5046
- Target namePCSK6
- Target descriptionproprotein convertase subtilisin/kexin type 6
- Target synonymsPACE4; paired basic amino acid cleaving enzyme 4; paired basic amino acid cleaving system 4; proprotein convertase subtilisin/kexin type 6; SPC4; subtilisin/kexin-like protease PACE4; subtilisin-like proprotein convertase 4
- HostRabbit
- IsotypeIgG
- Protein IDP29122
- Protein NameProprotein convertase subtilisin/kexin type 6
- Scientific DescriptionBoster Bio Anti-PACE4/PCSK6 Antibody Picoband® catalog # PB9769. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203