Anti-PAI-1 [8H9D4]

Absolute Antibody

Anti-PAI-1 [8H9D4]

7

This antibody binds human PAI-1 at an epitope between amino acids 277 and 327, more particularly it binds residues between Arg300, Gln303 and Asp305 located on the loop between alpha-helix I and beta-strand 5A. This antibody cross-reacts with pig PAI-1 bu

This antibody is reported to have strong inhibitory effect towards PAI-1 activity. The cross reactivity of this antibody towards other PAI-1 than human was done using ELISA (PMID: 9048903). This antibody shifts the reaction to the substrate pathway without decreasing the rate-limiting constant for reactive center loop insertion by tPA (PMID: 12223472). This antibody interferes with the final locking step in the serpin/proteinase interaction, thereby explaining its substrate inducing properties (PMID: 12871373).

ELISA, Neutralisation/Blocking

Inhibit; neutralize; ELISA

Research Use Only

Monoclonal

8H9D4

SERPINE1

serpin family E member 1

endothelial plasminogen activator inhibitor; PAI; PAI1; PAI-1; PLANH1; plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Rabbit

IgG

Plasminogen activator inhibitor 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Porcine

Human, pig

The original antibody was generated using PAI-1 complex as an immunogen.

-20°C,2°C to 8°C

12352203