Anti-PAI-1 [MEDI-579]

Absolute Antibody

Anti-PAI-1 [MEDI-579]

9

This antibody binds the reactive center loop (RCL) of human PAI-1 and at the same exosite used by both tissue and urokinase plasminogen activators (tPA and uPA). This antibody binds the active form of human PAI-1. Plasminogen activator inhibitor-1 (PAI-1)

This antibody specifically inhibits serine protease interactions with PAI-1 while conserving vitronectin binding. The binding of this antibody to native and latent form of human PAI-1 was determined using ELISA. This antibody was able to neutralize recombinant human, rat and mouse PAI-1 activity in species-relevant tPA-coupled chromogenic assays. The Fab version of this antibody binds human and rat PAI-1 with a binding affinity (Kd) of 5.34 pM and 84 pM respectively. Competition binding experiments also demonstrates that MEDI-579 does not interact with other circulating serpins (PMID: 30733557). This antibody is effective in slowing the progression of diabetic nephropathy in db/db mice and that the effect is additive to ACEI (PMID: 27511457).

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

MEDI-579

SERPINE1

serpin family E member 1

endothelial plasminogen activator inhibitor; PAI; PAI1; PAI-1; PLANH1; plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Rabbit

IgG

Plasminogen activator inhibitor 1

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203