Anti-PAR2 [Ab4999 (Par-B)]

Absolute Antibody

Anti-PAR2 [Ab4999 (Par-B)]

7

This antibody has binding specificity for N-terminal PAR-2 peptide SKGRSLIGKVDGTSHVTGKGV and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.

This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

ELISA

ELISA

Research Use Only

Monoclonal

Ab4999 (Par-B)

F2RL1

F2R like trypsin receptor 1

coagulation factor II (thrombin) receptor-like 1; GPR11; G-protein coupled receptor 11; PAR2; protease-activated receptor 2; proteinase-activated receptor 2; thrombin receptor-like 1

Human

IgG4

Proteinase-activated receptor 2

Human, Monkey, Mouse, Rat

Human, mouse, rat, cynomolgus monkey

The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.

-20°C,2°C to 8°C

12352203