Figure 1. Western blot analysis of PAR2 using anti-PAR2 antibody (PB9700). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: SW620 Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # PB9700) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 49 kDa. The expected band size for PAR2 is at 44 kDa.
Figure 1. Western blot analysis of PAR2 using anti-PAR2 antibody (PB9700). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: SW620 Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # PB9700) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 49 kDa. The expected band size for PAR2 is at 44 kDa.
Anti-PAR2/F2RL1 Antibody Picoband(r)
PB9700
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetF2RL1
Overview
- SupplierBoster Bio
- Product NameAnti-PAR2 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2150
- Target nameF2RL1
- Target descriptionF2R like trypsin receptor 1
- Target synonymscoagulation factor II (thrombin) receptor-like 1; GPR11; G-protein coupled receptor 11; PAR2; protease-activated receptor 2; proteinase-activated receptor 2; thrombin receptor-like 1
- HostRabbit
- IsotypeIgG
- Protein IDP55085
- Protein NameProteinase-activated receptor 2
- Scientific DescriptionBoster Bio Anti-PAR2/F2RL1 Antibody Picoband® catalog # PB9700. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203