Anti-Parathyroid Hormone-related Protein [23-57-137-1]

Absolute Antibody

Anti-Parathyroid Hormone-related Protein [23-57-137-1]

9

The Antibody binds to human PTHrP (The KD ~ 0.1 nM) at a site closely overlapping with the GPCR parathyroid hormone receptor 1 binding site - the epitope is between residues 20 and 30, with some contribution from residues 15-20 (aa 14-34 are important for

The antibody blocks the binding of PTHrP to parathyroid hormone receptor 1 through binding to a site closely overlapping with the receptor-interacting site at the extracellular loop and juxtamembrane regions. The antibody does not block the action of parathyroid hormone (PTH) through the same receptor. The antibody inhibits the action of PTHrP-(1-34) in promoting cAMP production by osteosarcoma cells by 90%,. The antibody also functions in vivo, and can treat nude mice rendered hypercalcemic by PAN-7-JCK cells, and prevents cachexia in mice bearing the LC6-JCK human lung cancer xenograft, independent of the calcium-lowering effect of the antibody. The fab fragment of this antibody, generated by papain digestion, has been used for cystallization of PTHrP at atomic level resolution.

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

23-57-137-1

PTHLH

parathyroid hormone like hormone

BDE2; HHM; osteostatin; parathyroid hormone-like hormone preproprotein; parathyroid hormone-like related protein; parathyroid hormone-related protein; PLP; PTHR; PTH-related protein; PTHRP; PTH-rP

Human

IgG1

Parathyroid hormone-related protein

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203