IHC analysis of PCNA using anti-PCNA antibody (MA1083). PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
IHC analysis of PCNA using anti-PCNA antibody (MA1083). PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-PCNA Antibody (Monoclonal, PC 10)
MA1083
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetPCNA
Overview
- SupplierBoster Bio
- Product NameAnti-PCNA Antibody (Monoclonal, PC 10)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteOther applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, IHF, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDPC 10
- Concentration100 ug/ml
- FormulationLyophilized
- Gene ID5111
- Target namePCNA
- Target descriptionproliferating cell nuclear antigen
- Target synonymsATLD2; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen
- HostMouse
- IsotypeIgG2a
- Protein IDP04961
- Protein NameProliferating cell nuclear antigen
- Scientific DescriptionBoster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityBovine, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
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- p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation. Li N et al., 2021, Stem Cells IntRead more
- MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway. Wang D et al., 2021 Dec 7, Cancer Cell IntRead more
- The promoting role of Cx43 on the proliferation and migration of arterial smooth muscle cells for angiotensin II-dependent hypertension. Gao RJ et al., 2021 Oct, Pulm Pharmacol TherRead more
- Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling. Zhang M et al., 2021, Stem Cells IntRead more
- The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-beta and PD-L1. Yi M et al., 2021 Feb 16, J Hematol OncolRead more
- Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Wei YD et al., 2021 Jan 18, Zool ResRead more
- Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system. Zhao Y et al., 2020 Aug, Exp Ther MedRead more