Anti-PD-1 [JC053]

Absolute Antibody

Anti-PD-1 [JC053]

9

This antibody binds canine PD-1.

This antibody is recommended for detection of canine PD-1. This antibody can detect canine PD-1 in a western blot assay. JC053 successfully detected canine PD-1 expressed on formalin-fixed paraffin-embedded (FFPE) tonsil and lymph node via immunohistochemistry suggesting that this could be useful for histological detection of canine PD-1 and as diagnostic tool in tumor biopsies. JC053 was also used to determine the PD-1 expression on CD4+ and CD8+ T-cells of canine PBMCs stimulated with ConA using flow cytometry. It could also detect recombinant canine PD-1 expression on CHO and CLGL-90 cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-gamma level. This antibody did not effectively block PD-1 and did not cause a recovery of T cell function (PMID: 32614928).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

JC053

Canine

IgG2

This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.

Canine

-20°C,2°C to 8°C

12352203